Mutation detection with myT BRAF consists of two steps:

1. Locus-specific qPCR
   A non-allele-specific qPCR is performed to assess total (mutant + wild-type) amplifiable BRAF for each sample. This determines the quantity of DNA to be used in step 2 for each sample
   
   
2. Allele-specific qPCR
   A mutant allele-specific BRAF qPCR is then performed to assess presence of V600E or V600K. This assay DOES distinguish between wild-type and mutant but DOES NOT distinguish between the two mutant alleles. Results are reported as positive or negative for mutant BRAF for each sample with a sensitivity limit of 1%. Samples containing less than 1% mutant BRAF are either negative or below the detection limit of this assay.

myT BRAF Workflow

Isolate genomic DNA from samples

Obtain UV absorbance readings

Perform locus-specific qPCR

Determine amplifiable copy number from Ct values

Perform allele-specific qPCR

Determine BRAF V600E/K status

Reagents Included

Shipped in a separate box:

Reagents Not included

The following reagents are not provided and must be ordered separately from a licensed vendor. The purchase price of the probe and shipping costs are included in your purchase of myT BRAF. Instructions on how to easily order the probe from our recommended vendor and a coupon covering the purchase price and shipping cost of the probe will be provided with your order acknowledgement. This will enable you to synchronize delivery of the probe with delivery of myT BRAF. The cost of master mix purchase is not included.

Number of Reactions

The product contents are sufficient to perform 60 reactions consisting of 30 locus-specific and 30 allele-specific reactions. This enables testing of up to 28 samples when including a positive control and a no-template control (NTC) when performed as a single qPCR run. If testing is split into multiple batches, total samples tested will be less as a positive control and NTC are required for each run. For example, if testing is batched into 3 qPCR runs, the total number of samples analyzed will be 24, in addition to 3 positive control and 3 NTC reactions.

Scientific Posters on myT BRAF

To obtain a copy of one of our posters from recent scientific meetings, please go to the Contact page and request the specific poster(s):

Sensitive and specific detection of BRAF and KRAS mutations with myT primers

Single copy detection of KRAS mutations in cancer tissue and blood by qPCR using primers with enhanced selectivity

Single copy qPCR-based detection of KRAS and BRAF mutations in cancer

Single copy qPCR-based detection of BRAF and KRAS mutations

Material Safety Data Sheets

To download PDF versions of Material Safety Data Sheets (MSDS), please click on the relevant reagent below:

MSDS SBI-0002 BRAF Allele-specific Primer Mix

MSDS SBI-0003 BRAF Locus-specific Primer Mix

MSDS SBI-0004 DNA Standard

MSDS SBI-0005 Nuclease-free Buffer